Everything about Silver Stain totally explained
Silver staining is the use of
silver to stain
histologic sections. This kind of staining is important especially to show
proteins (for example type III
collagen) and
DNA. It is used to show both substances inside and outside
cells. Silver staining is also used in
temperature gradient gel electrophoresis and in
polyacrylamide gels.
Some cells are
argentaffin. These
reduce silver solution to metallic silver after
formalin fixation. Other cells are
argyrophilic. These reduce silver solution to metallic silver after being exposed to the stain that contains a
reductant, for example
hydroquinone or formalin.
Silver nitrate forms insoluble
silver phosphate with
phosphate ions. When subjected to a reducing agent, usually
hydroquinone, it forms black elementary silver. This is used for study of formation of
calcium phosphate particles during
bone growth.
Silver staining is used in
light microscopy. The metallic silver particles are deposited on sensitised reticulin fibres and are then easily seen in the microscopic preparations.
Silver stain aids in the perception of
reticular fibers.
Use in art
Silver staining is also a technique in traditional
stained glass to produce the yellow, brown or amber shading when painting on glass. It is a technique that's often used for realistic hair colors. It was discovered in the
14th Century but wasn't originally used very frequently.
Further Information
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